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rabbit anti human eif2α  (Proteintech)


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    Proteintech rabbit anti human eif2α
    Antibodies used for Western blots.
    Rabbit Anti Human Eif2α, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 108 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+human+eif2%CE%B1/EIF2S1%2FEIF2A+Antibody/pmc11977284-6-0-4
    Average 95 stars, based on 108 article reviews
    rabbit anti human eif2α - by Bioz Stars, 2026-09
    95/100 stars

    Images

    1) Product Images from "Combination of brefeldin A and tunicamycin induces apoptosis in HepG2 cells through the endoplasmic reticulum stress-activated PERK-eIF2α-ATF4-CHOP signaling pathway"

    Article Title: Combination of brefeldin A and tunicamycin induces apoptosis in HepG2 cells through the endoplasmic reticulum stress-activated PERK-eIF2α-ATF4-CHOP signaling pathway

    Journal: Liver Research

    doi: 10.1016/j.livres.2025.01.004

    Antibodies used for Western blots.
    Figure Legend Snippet: Antibodies used for Western blots.

    Techniques Used: Western Blot

    Primers of the genes used for qRT-PCR.
    Figure Legend Snippet: Primers of the genes used for qRT-PCR.

    Techniques Used: Sequencing

    Effects of BFA and TM on the levels of ER stress-related proteins (BiP, PERK, eIF2α, ATF4, and CHOP). HepG2 cells were treated with BFA (0.25 mg/L) and TM (1 mg/L), either individually or in combination, for 24 h. The protein levels of (A) BiP, (B) PERK, (C) phosphorylated PERK (p-PERK), (D) eIF2α, (E) phosphorylated eIF2α (p-eIF2α), (F) ATF4, and (G) CHOP were determined by Western blotting. Data are presented as means ± SD from three independent experiments performed in triplicate. ∗ P < 0.05, compared to the control group; # P < 0.05, compared to the DMSO (0.1% v/v, vehicle control) group; a P < 0.05, compared to the BFA (0.25 mg/L) group. Abbreviations: ATF4, activating transcription factor 4; BFA, brefeldin A; BiP, binding immunoglobulin protein; CHOP, C/EBP homologous protein; DMSO, dimethyl sulfoxide; eIF2α, eukaryotic translation initiation factor 2α; PERK, protein kinase R-like endoplasmic reticulum kinase; SD, standard deviation; TM, tunicamycin.
    Figure Legend Snippet: Effects of BFA and TM on the levels of ER stress-related proteins (BiP, PERK, eIF2α, ATF4, and CHOP). HepG2 cells were treated with BFA (0.25 mg/L) and TM (1 mg/L), either individually or in combination, for 24 h. The protein levels of (A) BiP, (B) PERK, (C) phosphorylated PERK (p-PERK), (D) eIF2α, (E) phosphorylated eIF2α (p-eIF2α), (F) ATF4, and (G) CHOP were determined by Western blotting. Data are presented as means ± SD from three independent experiments performed in triplicate. ∗ P < 0.05, compared to the control group; # P < 0.05, compared to the DMSO (0.1% v/v, vehicle control) group; a P < 0.05, compared to the BFA (0.25 mg/L) group. Abbreviations: ATF4, activating transcription factor 4; BFA, brefeldin A; BiP, binding immunoglobulin protein; CHOP, C/EBP homologous protein; DMSO, dimethyl sulfoxide; eIF2α, eukaryotic translation initiation factor 2α; PERK, protein kinase R-like endoplasmic reticulum kinase; SD, standard deviation; TM, tunicamycin.

    Techniques Used: Western Blot, Control, Binding Assay, Standard Deviation

    Effects of BFA and TM on the mRNA levels of ER stress-related genes ( BiP, PERK, eIF2α, ATF4, and CHOP ). HepG2 cells were treated with BFA (0.25 mg/L) and TM (1 mg/L), either individually or in combination, for 24 h. The relative mRNA levels of (A) BiP , (B) PERK , (C) eIF2α, (D) ATF4 , and (E) CHOP were determined by qRT-PCR. Data are presented as means ± SD from three independent experiments performed in triplicate. ∗ P < 0.05, compared to the control group; # P < 0.05, compared to the DMSO (0.1% v/v, vehicle control) group; a P < 0.05, compared to the BFA (0.25 mg/L) group. Abbreviations: ATF4, activating transcription factor 4; BFA, brefeldin A; BiP, binding immunoglobulin protein; CHOP, C/EBP homologous protein; DMSO, dimethyl sulfoxide; eIF2α, eukaryotic translation initiation factor 2α; PERK, protein kinase R-like endoplasmic reticulum kinase; SD, standard deviation; TM, tunicamycin.
    Figure Legend Snippet: Effects of BFA and TM on the mRNA levels of ER stress-related genes ( BiP, PERK, eIF2α, ATF4, and CHOP ). HepG2 cells were treated with BFA (0.25 mg/L) and TM (1 mg/L), either individually or in combination, for 24 h. The relative mRNA levels of (A) BiP , (B) PERK , (C) eIF2α, (D) ATF4 , and (E) CHOP were determined by qRT-PCR. Data are presented as means ± SD from three independent experiments performed in triplicate. ∗ P < 0.05, compared to the control group; # P < 0.05, compared to the DMSO (0.1% v/v, vehicle control) group; a P < 0.05, compared to the BFA (0.25 mg/L) group. Abbreviations: ATF4, activating transcription factor 4; BFA, brefeldin A; BiP, binding immunoglobulin protein; CHOP, C/EBP homologous protein; DMSO, dimethyl sulfoxide; eIF2α, eukaryotic translation initiation factor 2α; PERK, protein kinase R-like endoplasmic reticulum kinase; SD, standard deviation; TM, tunicamycin.

    Techniques Used: Quantitative RT-PCR, Control, Binding Assay, Standard Deviation

    Related Articles

    Western Blot:


    Sequencing:


    Control:


    Binding Assay:


    Standard Deviation:


    Quantitative RT-PCR:




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    Image Search Results


    Fig. 1 a: BCL2 expression: 80% (DABx200) b: Beclin-1 expression: 100% (DABx200) c–e: eIF2α expression with vary ing intensities: weak (+ 1) in (c), moderate (+ 2) in (d), and strong (+ 3) in (e). (DABx200)

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    Figure Lengend Snippet: Fig. 1 a: BCL2 expression: 80% (DABx200) b: Beclin-1 expression: 100% (DABx200) c–e: eIF2α expression with vary ing intensities: weak (+ 1) in (c), moderate (+ 2) in (d), and strong (+ 3) in (e). (DABx200)

    Article Snippet: The following primary antibodies were used for immunohistochemical staining: monoclonal mouse anti-human BCL2 (clone 124, prediluted, Dako), monoclonal rabbit anti-human Beclin-1 (clone EP304, 1:100, Epitomics), monoclonal rabbit anti-human HIF-1 Alpha (clone EP118, 1:100, Epitomics), monoclonal rabbit anti-human eIF2α (clone D7D3, 1:800, Cell Signaling), and monoclonal rabbit anti-human LC3A (clone D50G8, 1:3000, Cell Signaling).

    Techniques: Expressing

    Antibodies used for Western blots.

    Journal: Liver Research

    Article Title: Combination of brefeldin A and tunicamycin induces apoptosis in HepG2 cells through the endoplasmic reticulum stress-activated PERK-eIF2α-ATF4-CHOP signaling pathway

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    Figure Lengend Snippet: Antibodies used for Western blots.

    Article Snippet: Rabbit anti-human eIF2α , Protein Tech, China , 11170-1-AP , 1:1000.

    Techniques: Western Blot

    Primers of the genes used for qRT-PCR.

    Journal: Liver Research

    Article Title: Combination of brefeldin A and tunicamycin induces apoptosis in HepG2 cells through the endoplasmic reticulum stress-activated PERK-eIF2α-ATF4-CHOP signaling pathway

    doi: 10.1016/j.livres.2025.01.004

    Figure Lengend Snippet: Primers of the genes used for qRT-PCR.

    Article Snippet: Rabbit anti-human eIF2α , Protein Tech, China , 11170-1-AP , 1:1000.

    Techniques: Sequencing

    Effects of BFA and TM on the levels of ER stress-related proteins (BiP, PERK, eIF2α, ATF4, and CHOP). HepG2 cells were treated with BFA (0.25 mg/L) and TM (1 mg/L), either individually or in combination, for 24 h. The protein levels of (A) BiP, (B) PERK, (C) phosphorylated PERK (p-PERK), (D) eIF2α, (E) phosphorylated eIF2α (p-eIF2α), (F) ATF4, and (G) CHOP were determined by Western blotting. Data are presented as means ± SD from three independent experiments performed in triplicate. ∗ P < 0.05, compared to the control group; # P < 0.05, compared to the DMSO (0.1% v/v, vehicle control) group; a P < 0.05, compared to the BFA (0.25 mg/L) group. Abbreviations: ATF4, activating transcription factor 4; BFA, brefeldin A; BiP, binding immunoglobulin protein; CHOP, C/EBP homologous protein; DMSO, dimethyl sulfoxide; eIF2α, eukaryotic translation initiation factor 2α; PERK, protein kinase R-like endoplasmic reticulum kinase; SD, standard deviation; TM, tunicamycin.

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    Article Title: Combination of brefeldin A and tunicamycin induces apoptosis in HepG2 cells through the endoplasmic reticulum stress-activated PERK-eIF2α-ATF4-CHOP signaling pathway

    doi: 10.1016/j.livres.2025.01.004

    Figure Lengend Snippet: Effects of BFA and TM on the levels of ER stress-related proteins (BiP, PERK, eIF2α, ATF4, and CHOP). HepG2 cells were treated with BFA (0.25 mg/L) and TM (1 mg/L), either individually or in combination, for 24 h. The protein levels of (A) BiP, (B) PERK, (C) phosphorylated PERK (p-PERK), (D) eIF2α, (E) phosphorylated eIF2α (p-eIF2α), (F) ATF4, and (G) CHOP were determined by Western blotting. Data are presented as means ± SD from three independent experiments performed in triplicate. ∗ P < 0.05, compared to the control group; # P < 0.05, compared to the DMSO (0.1% v/v, vehicle control) group; a P < 0.05, compared to the BFA (0.25 mg/L) group. Abbreviations: ATF4, activating transcription factor 4; BFA, brefeldin A; BiP, binding immunoglobulin protein; CHOP, C/EBP homologous protein; DMSO, dimethyl sulfoxide; eIF2α, eukaryotic translation initiation factor 2α; PERK, protein kinase R-like endoplasmic reticulum kinase; SD, standard deviation; TM, tunicamycin.

    Article Snippet: Rabbit anti-human eIF2α , Protein Tech, China , 11170-1-AP , 1:1000.

    Techniques: Western Blot, Control, Binding Assay, Standard Deviation

    Effects of BFA and TM on the mRNA levels of ER stress-related genes ( BiP, PERK, eIF2α, ATF4, and CHOP ). HepG2 cells were treated with BFA (0.25 mg/L) and TM (1 mg/L), either individually or in combination, for 24 h. The relative mRNA levels of (A) BiP , (B) PERK , (C) eIF2α, (D) ATF4 , and (E) CHOP were determined by qRT-PCR. Data are presented as means ± SD from three independent experiments performed in triplicate. ∗ P < 0.05, compared to the control group; # P < 0.05, compared to the DMSO (0.1% v/v, vehicle control) group; a P < 0.05, compared to the BFA (0.25 mg/L) group. Abbreviations: ATF4, activating transcription factor 4; BFA, brefeldin A; BiP, binding immunoglobulin protein; CHOP, C/EBP homologous protein; DMSO, dimethyl sulfoxide; eIF2α, eukaryotic translation initiation factor 2α; PERK, protein kinase R-like endoplasmic reticulum kinase; SD, standard deviation; TM, tunicamycin.

    Journal: Liver Research

    Article Title: Combination of brefeldin A and tunicamycin induces apoptosis in HepG2 cells through the endoplasmic reticulum stress-activated PERK-eIF2α-ATF4-CHOP signaling pathway

    doi: 10.1016/j.livres.2025.01.004

    Figure Lengend Snippet: Effects of BFA and TM on the mRNA levels of ER stress-related genes ( BiP, PERK, eIF2α, ATF4, and CHOP ). HepG2 cells were treated with BFA (0.25 mg/L) and TM (1 mg/L), either individually or in combination, for 24 h. The relative mRNA levels of (A) BiP , (B) PERK , (C) eIF2α, (D) ATF4 , and (E) CHOP were determined by qRT-PCR. Data are presented as means ± SD from three independent experiments performed in triplicate. ∗ P < 0.05, compared to the control group; # P < 0.05, compared to the DMSO (0.1% v/v, vehicle control) group; a P < 0.05, compared to the BFA (0.25 mg/L) group. Abbreviations: ATF4, activating transcription factor 4; BFA, brefeldin A; BiP, binding immunoglobulin protein; CHOP, C/EBP homologous protein; DMSO, dimethyl sulfoxide; eIF2α, eukaryotic translation initiation factor 2α; PERK, protein kinase R-like endoplasmic reticulum kinase; SD, standard deviation; TM, tunicamycin.

    Article Snippet: Rabbit anti-human eIF2α , Protein Tech, China , 11170-1-AP , 1:1000.

    Techniques: Quantitative RT-PCR, Control, Binding Assay, Standard Deviation

    Antibodies used for Western blots.

    Journal: Liver Research

    Article Title: Combination of brefeldin A and tunicamycin induces apoptosis in HepG2 cells through the endoplasmic reticulum stress-activated PERK-eIF2α-ATF4-CHOP signaling pathway

    doi: 10.1016/j.livres.2025.01.004

    Figure Lengend Snippet: Antibodies used for Western blots.

    Article Snippet: Rabbit anti-human Phospho-eIF2α , CST, USA , 3398T , 1:1000.

    Techniques: Western Blot

    Western blot analysis demonstrates the effect of 48 h GDC-0980 treatment on the expression of PERK, EiF2α, and P-EiF2α Ser51 in A-172 and U-118 MG glioblastoma cells, using β-tubulin as a protein-loading control. Samples containing 20 μg of protein were submitted to electrophoresis and immunoblotting. The representative bands of the PERK, EiF2α, and P-EiF2α Ser51 to β-tubulin are illustrated. Data are presented as the means ± SEM. * p < 0.05.

    Journal: International Journal of Molecular Sciences

    Article Title: Novel Dual PI3K/mTOR Inhibitor, Apitolisib (GDC-0980), Inhibits Growth and Induces Apoptosis in Human Glioblastoma Cells

    doi: 10.3390/ijms222111511

    Figure Lengend Snippet: Western blot analysis demonstrates the effect of 48 h GDC-0980 treatment on the expression of PERK, EiF2α, and P-EiF2α Ser51 in A-172 and U-118 MG glioblastoma cells, using β-tubulin as a protein-loading control. Samples containing 20 μg of protein were submitted to electrophoresis and immunoblotting. The representative bands of the PERK, EiF2α, and P-EiF2α Ser51 to β-tubulin are illustrated. Data are presented as the means ± SEM. * p < 0.05.

    Article Snippet: Monoclonal (rabbit) anti-human CHOP antibody, polyclonal (rabbit) anti-human PARP antibody, polyclonal (rabbit) anti-human cleaved caspase-3 antibody, monoclonal (mouse) anti-human cleaved caspase-8 antibody, monoclonal (rabbit) anti-human cleaved caspase-9 antibody, monoclonal (rabbit) anti-human DR-5 antibody, polyclonal (rabbit) anti-human BAX antibody, monoclonal (rabbit) anti-human BCL-2 antibody, monoclonal (rabbit) anti-human PERK antibody, polyclonal (rabbit) anti-human EIF2α antibody, monoclonal (rabbit) anti-human Phospho-EIF2α Ser51 antibody, polyclonal (rabbit) anti-human Phospho-p70S6K Thr421/Ser424 antibody, monoclonal (rabbit) anti-human Phospho-AKT Ser473 antibody, monoclonal (rabbit) anti-human Phospho-mTOR Ser2448 antibody, polyclonal (rabbit) anti-human LC3A/B antibody, polyclonal (rabbit) anti-human β-tubulin antibody, and alkaline phosphatase-labeled anti-rabbit immunoglobulin G were provided by Cell Signaling Technology (Boston, MA, USA).

    Techniques: Western Blot, Expressing, Control, Electrophoresis

    Western blot analysis demonstrates the effect of 48 h GDC-0980 treatment on the expression of PERK, EiF2α, and P-EiF2α Ser51 in A-172 and U-118 MG glioblastoma cells, using β-tubulin as a protein-loading control. Samples containing 20 μg of protein were submitted to electrophoresis and immunoblotting. The representative bands of the PERK, EiF2α, and P-EiF2α Ser51 to β-tubulin are illustrated. Data are presented as the means ± SEM. * p < 0.05.

    Journal: International Journal of Molecular Sciences

    Article Title: Novel Dual PI3K/mTOR Inhibitor, Apitolisib (GDC-0980), Inhibits Growth and Induces Apoptosis in Human Glioblastoma Cells

    doi: 10.3390/ijms222111511

    Figure Lengend Snippet: Western blot analysis demonstrates the effect of 48 h GDC-0980 treatment on the expression of PERK, EiF2α, and P-EiF2α Ser51 in A-172 and U-118 MG glioblastoma cells, using β-tubulin as a protein-loading control. Samples containing 20 μg of protein were submitted to electrophoresis and immunoblotting. The representative bands of the PERK, EiF2α, and P-EiF2α Ser51 to β-tubulin are illustrated. Data are presented as the means ± SEM. * p < 0.05.

    Article Snippet: Monoclonal (rabbit) anti-human CHOP antibody, polyclonal (rabbit) anti-human PARP antibody, polyclonal (rabbit) anti-human cleaved caspase-3 antibody, monoclonal (mouse) anti-human cleaved caspase-8 antibody, monoclonal (rabbit) anti-human cleaved caspase-9 antibody, monoclonal (rabbit) anti-human DR-5 antibody, polyclonal (rabbit) anti-human BAX antibody, monoclonal (rabbit) anti-human BCL-2 antibody, monoclonal (rabbit) anti-human PERK antibody, polyclonal (rabbit) anti-human EIF2α antibody, monoclonal (rabbit) anti-human Phospho-EIF2α Ser51 antibody, polyclonal (rabbit) anti-human Phospho-p70S6K Thr421/Ser424 antibody, monoclonal (rabbit) anti-human Phospho-AKT Ser473 antibody, monoclonal (rabbit) anti-human Phospho-mTOR Ser2448 antibody, polyclonal (rabbit) anti-human LC3A/B antibody, polyclonal (rabbit) anti-human β-tubulin antibody, and alkaline phosphatase-labeled anti-rabbit immunoglobulin G were provided by Cell Signaling Technology (Boston, MA, USA).

    Techniques: Western Blot, Expressing, Control, Electrophoresis